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The Embryo Biopsy Procedure - A View Under The Micromanipulator

Fig 1. 7-cell embryo, on Day 3 after IVF, ready
to be biopsied. It is held in place on a micromanipulator with a holding pipette.

Fig 2. The laser is used to create a hole through the shell
 ( the zona) of the embryo

Fig 3. The embryo biopsy pipette is introduced through this opening, and gentle suction is applied to dislodge a single cell ( a blastomere ) from the embryo .

Fig 4. The blastomere is being removed. You can see the nucleus of the blastomere ( the clear area in the center of the cell) clearly. This contains the DNA of the cell.

Fig 5. The embryo biopsy has been completed
successfully !

Fig 6. The biopsied embryo is intact after the biopsy has been completed. This procedure is repeated for all the embryos.

Fig 7. The biopsied embryo is now returned to the incubator for further culture. The blastomere will now be analysed in the FISH lab.

Fig 8. The FISH probes are available as a "ready-to-use" kit from Vysis, USA.


Fig 9. FISH signals from a normal embryo. We know these are from a male ( XY) embryo as each has a orange signal 
( orange dot) representing a single X chromosome, and a green signal, representing a single Y chromosome.

Once the results are known, which takes about 4-6 hours, only the normal embryos will be replaced into the mother's uterus.


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  IN THE NEWS

 
   
 
Outlook Magazine
ranks
Malpani Infertility
Clinic
among the top five
IVF clinics in India.


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  Dr Malpani, Malpani Infertility Clinic.
Jamuna Sagar, Shahid Bhagat Singh Road, Colaba, Bombay 400 005.
Tel: 91-22-22151065, 91-22-22151066 Fax (India): 91-22-2215 0223

Email:
info@drmalpani.com