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Dr. Sai, Embryologist informs you how we freeze your sperms and what are the steps involved during freezing the semen.
Dr. Sai, Embryologist, Malpani Infertility Clinic
The nurse will ask you to write your full name on a piece of paper, to avoid any spelling mistakes . This name will be written on the semen collection jar.
This is a sterile plastic clean large mouthed jar. This is labeled with your name and the cap of the jar is labeled with your wife's name. Once the sample is collected, it arrives in the lab along with your file.The name on the file is cross checked with the one on the jar.
Your semen sample is assessed for Volume of sample, Sperm Count, and percentage of motile sperms.
Your sperm are stored in straws. These have a capacity of 0.5ml or 0.25 ml. If we are freezing your sperms for short term period, we freeze them in 0.5ml straws. If it's a long term freeze, we freeze them in 0.25 ml straws, so that we have more straws to use.
The straws are labeled with an ID number which will be unique for you. The ID number is generally a 6 digit number. The labeling is done corresponding to a Cm Scale as shown in the figure. This is called the French labeling system.
The sperm are delicate. To protect them from damage caused by low temperatures
( we freeze them at -196 C) , we add cryoprotectant medium to your sample.
Your sperm sample is loaded into your labeled straws with the help of a syringe.
The loaded straws are sealed with sealing powder. Sealing powder come in different
colors. For freezing different patient's sperms we use different colored powder for
sealing. This colour coding helps us to identify samples with ease.
Once your straws are sealed, they are placed in visitube. Visitubes come in different
Colors as well. Different colored visitubes are used for different patients.
Loaded into Labelled Straws
Straws are kept in Visitubes
Visitubes are kept in Canisters
The Sperm freezing procedure is a very complex one and involves a number of steps. Experts at the Malpani Infertility Clinic handle this job expertly and meticulously.