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Here are some images of the embryo biopsy procedure under a micromanipulator. The images are reproduced stage by stage to help you get an exact idea about how things work. As you can see, this is a delicate operation, and needs to be done by a skilled embryologist. It's very easy for a clumsy technician to kill your precious embryos when doing the biopsy, so make sure you find a good IVF clinic !
Read more- Embryo Biopsy for PGD
Fig 1. 7-cell embryo, on Day 3 after IVF, ready to be biopsied. It is held in place on a micromanipulator with a holding pipette.
Fig 2. The laser is used to create a hole through the shell ( the zona) of the embryo
Fig 3. The embryo biopsy pipette is introduced through this opening, and gentle suction is applied to dislodge a single cell ( a blastomere ) from the embryo .
Fig 4. The blastomere is being removed. You can see the nucleus of the blastomere ( the clear area in the center of the cell) clearly. This contains the DNA of the cell.
Fig 5. The embryo biopsy has been completed successfully !
Fig 6. The biopsied embryo is intact after the biopsy has been completed. This procedure is repeated for all the embryos.
Fig 7. The biopsied embryo is now returned to the incubator for further culture. The blastomere will now be analysed in the FISH lab.
Fig 9. FISH signals from a normal embryo. We know these are from a male ( XY) embryo as each has a orange signal ( orange dot) representing a single X chromosome, and a green signal, representing a single Y chromosome.
Once the results are known, which takes about 4-6 hours, only the normal embryos will be replaced into the mother's uterus. The FISH probes are available as a "ready-to-use" kit from Vysis, USA.
Read more- PGD - Preimplantation Genetic Diagnosis The Newest ART
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